Author: Michael Greenberg

In collaboration with the Gurnett and Johnson labs, we looked at pathogenic mutations in tropomyosin that cause various forms of skeletal myopathies and birth defects. McAdow J, Yang S, Ou T, Huang G, Dobbs MB, Gurnett CA, Greenberg MJ, Johnson AN. A pathogenic mechanism associated with myopathies and structural birth defects involves TPM2 directed myogenesis. JCI Insight. 2022 May 17:e152466. doi: 10.1172/jci.insight.152466. Epub ahead of print. PMID: 35579956.

The Greenberg lab had 4 presentations at BPS 2022: Platform (Samantha Barrick - Postdoc): STRUCTURAL DIFFERENCES IN VINCULIN AND METAVINCULIN ACTIN-BINDING DOMAINS EXPLORED BY MOLECULAR DYNAMICS SIMULATIONS Poster (Jeff Lotthammer – Student Bowman lab): EXPLORING THE MYOSIN ACTIVE/AUTO-INHIBITED STATE EQUILIBRIUM BY MARKOV STATE MODELING Poster (Artur Meller – Student Bowman lab): SIGNATURES OF ALLOSTERIC MODULATOR SPECIFICITY ARE ENCODED IN MYOSIN MOTOR DOMAIN EQUILIBRIUM FLUCTUATIONS Poster (Michael Greenberg - PI): HARNESSING MULTISCALE MODELS OF A DILATED CARDIOMYOPATHY MUTATION FOR PRECISION MEDICINE

The Greenberg lab had 2 posters as ASCB 2021: Dr. Samantha Barrick presented a poster: A Troponin T Variant Linked with Pediatric Dilated Cardiomyopathy Decreases Cardiac Contractility by Reducing the Coupling of Thin Filament Activation to Myosin and Calcium Binding And Michael Greenberg presented: Utilizing Multiscale Models of a Dilated Cardiomyopathy Mutation for Precision Medicine, which included collaborative work from the Lavine lab.

This article, co-authored with Dr. Samantha Barrick, focuses on cardiac myosin, highlighting new regulatory mechanisms, its roles beyond sarcomeric contraction, its emergence as a drug target, and some outstanding questions for the field. The article can be found here.

In collaboration with the labs of Drs. Samantha Harris, Stuart Campbell, and Jonathan Kirk, the Greenberg lab published a paper on actomyosin dysfunction in diabetic heart failure. Congratulations to Dr. Samantha Barrick and our collaborators. The publication can here.

Congratulations to Dr. Sarah Clippinger Schulte for successfully defending her thesis. Dr. Schulte is the first Greenberg lab graduate student to receive their PhD.

A new publication in Immunity, including Greenberg lab contributions can be found here. This work stems from one of our long-standing collaborations with the Lavine lab. Congratulations to all of our co-authors.

New publication from the Greenberg lab is now online at the Molecular Biology of the Cell (here).  This work, led by first author Dr. Samantha Barrick, examines a variant in troponin T associated with pediatric onset dilated cardiomyopathy. We show that this variant causes molecular and cellular alterations consistent with those seen in known pathogenic variants. We show that the molecular mechanism driving the early disease pathogenesis is reduced coupling between calcium binding to troponin C and thin filament activation. This in turn leads to changes in cellular contractility and sarcomeric organization.

The preprint, describing our collaborative project with the labs of Samantha Harris, Stuart Campbell, and Jonathan Kirk can be found here.

See the cover here.