Recent Publications

For a complete list of Greenberg lab publications, click here :

 

Selected Publications with Hyperlinks (bold denotes Greenberg Lab members):

Garg A, Jansen S, Greenberg L, Zhang R, Lavine KJ, Greenberg MJ. Dilated cardiomyopathy-associated skeletal muscle actin (ACTA1) mutation R256H disrupts actin structure and function and causes cardiomyocyte hypocontractility. Proc Natl Acad Sci U S A. 2024 Nov 12;121(46):e2405020121. doi: 10.1073/pnas.2405020121. Epub 2024 Nov 6. PMID: 39503885.

Walker EC, Javati S, Todd EM, Matlam JP, Lin X, Bryant M, Krone E, Ramani R, Chandra P, Green TP, Anaya EP, Zhou JY, Alexander KA, Tong RS, Yuasi L, Boluarte S, Yang F, Greenberg L, Nerbonne JM, Greenberg MJ, Clemens RA, Philips JA, Wilson LD, Halabi CM, DeBosch BJ, Blyth CC, Druley TE, Kazura JW, Pomat WS, Morley SC. Novel coenzyme Q6 genetic variant increases susceptibility to pneumococcal disease. Nat Immunol. 2024 Nov 4. doi: 10.1038/s41590-024-01998-4. Epub ahead of print. PMID: 39496954.

Scott B, Greenberg L, Squarci C, Campbell KS, Greenberg MJ. Danicamtiv reduces myosin’s working stroke but enhances contraction by activating the thin filament. bioRxiv [Preprint]. 2024 Oct 11:2024.10.09.617269. doi: 10.1101/2024.10.09.617269. PMID: 39416013; PMCID: PMC11482770.

Greenberg MJ. Super-relaxed or super-stressed: Modeling length-dependent activation in cardiac muscle. Biophys J. 2024 Sep 17;123(18):2957-2960. doi: 10.1016/j.bpj.2024.07.033. Epub 2024 Jul 26. PMID: 39068513; PMCID: PMC11427768.

Lin Z, Xie Y, Gongora J, Liu X, Zahn E, Palai BB, Ramirez D, Searfoss RM, Vitorino FN, Dann GP, Zhao C, Han X, MacTaggart B, Lan X, Fu D, Greenberg L, Zhang Y, Lavine KJ, Greenberg MJ, Lv D, Kashina A, Garcia BA. An Unbiased Proteomic Platform for Activity-based Arginylation Profiling. bioRxiv [Preprint]. 2024 Jun 2:2024.06.01.596974. doi: 10.1101/2024.06.01.596974. PMID: 38854050; PMCID: PMC11160793.

Cubuk J, Greenberg L, Greenberg AE, Emenecker RJ, Stuchell-Brereton MD, Holehouse AS, Soranno A, Greenberg MJ. Structural dynamics of the intrinsically disordered linker region of cardiac troponin T. bioRxiv [Preprint]. 2024 May 31:2024.05.30.596451. doi: 10.1101/2024.05.30.596451. PMID: 38853835; PMCID: PMC11160775.

Greenberg L, Stump WT, Lin Z, Bredemeyer AL, Blackwell T, Han X, Greenberg AE, Garcia BA, Lavine KJ, Greenberg MJ. Harnessing molecular mechanism for precision medicine in dilated cardiomyopathy caused by a mutation in troponin T. bioRxiv [Preprint]. 2024 Apr 9:2024.04.05.588306. doi: 10.1101/2024.04.05.588306. PMID: 38645235; PMCID: PMC11030379.

Meyer GA, Ferey JLA, Sanford JA, Fitzgerald LS, Greenberg AE, Svensson K, Greenberg MJ, Schenk S. Insights into posttranslational regulation of skeletal muscle contractile function by the acetyltransferases, p300 and CBP. J Appl Physiol (1985). 2024 Jun 1;136(6):1559-1567. doi: 10.1152/japplphysiol.00156.2024. Epub 2024 May 9. PMID: 38722753; PMCID: PMC11365544.

Ghahremani S, Kanwal A, Pettinato A, Ladha F, Legere N, Thakar K, Zhu Y, Tjong H, Wilderman A, Stump WT, Greenberg L, Greenberg MJ, Cotney J, Wei CL, Hinson JT. CRISPR Activation Reverses Haploinsufficiency and Functional Deficits Caused by TTN Truncation Variants. Circulation. 2024 Apr 16;149(16):1285-1297. doi: 10.1161/CIRCULATIONAHA.123.063972. Epub 2024 Jan 18. PMID: 38235591; PMCID: PMC11031707.

Garg A, Lavine KJ, Greenberg MJ. Assessing Cardiac Contractility From Single Molecules to Whole Hearts. JACC Basic Transl Sci. 2023 Oct 11;9(3):414-439. doi: 10.1016/j.jacbts.2023.07.013. PMID: 38559627; PMCID: PMC10978360.

Scott B, Greenberg MJ. Multiscale biophysical models of cardiomyopathies reveal complexities challenging existing dogmas. Biophys J. 2023 Dec 19;122(24):4632-4634. doi: 10.1016/j.bpj.2023.11.014. Epub 2023 Nov 25. PMID: 38006882; PMCID: PMC10754685.

Lehman SJ, Meller A, Solieva SO, Lotthammer JM, Greenberg L, Langer SJ, Greenberg MJ, Tardiff JC, Bowman GR, Leinwand L. Divergent Molecular Phenotypes in Point Mutations at the Same Residue in Beta-Myosin Heavy Chain Lead to Distinct Cardiomyopathies. bioRxiv [Preprint]. 2023 Jul 3:2023.07.03.547580. doi: 10.1101/2023.07.03.547580. PMID: 37461648; PMCID: PMC10349964.

Clippinger-Schulte SR, Scott B, Barrick SK, Blackwell T, Stump WT, Greenberg MJ. Single molecule mechanics and kinetics of cardiac myosin interacting with regulated thin filaments.  Biophys J. 2023 Jun 20;122(12):2544-2555. doi: 10.1016/j.bpj.2023.05.008. Epub 2023 May 10. PMID: 37165621; PMCID: PMC10323011.

Lee LA, Barrick SK, Buvoli AE, Walklate J, Stump WT, Geeves M, Greenberg MJ, Leinwand LA. Distinct effects of two hearing loss-associated mutations in the sarcomeric myosin MYH7b. J Biol Chem. 2023 May;299(5):104631. doi: 10.1016/j.jbc.2023.104631. Epub 2023 Mar 22. PMID: 36963494; PMCID: PMC10141508.

Barrick SK, Garg A, Greenberg L, Zhang S, Lin CY, Stitziel NO, Greenberg MJ. Functional assays reveal the pathogenic mechanism of a de novo tropomyosin variant identified in patient with dilated cardiomyopathy. J Mol Cell Cardiol. 2023 Mar;176:58-67. doi: 10.1016/j.yjmcc.2023.01.014. Epub 2023 Feb 3. PMID: 36739943.

Meller A, Lotthammer JM, Smith LG, Novak B, Lee LA, Kuhn CC, Greenberg L, Leinwand LA, Greenberg MJ, Bowman GR. Drug specificity and affinity are encoded in the probability of cryptic pocket opening in myosin motor domains. Elife. 2023 Jan 27;12:e83602. doi: 10.7554/eLife.83602. PMID: 36705568; PMCID: PMC9995120.
Lee LA, Barrick SK, Meller A, Walklate J, Lotthammer JM, Tay JW, Stump WT, Bowman G, Geeves MA, Greenberg MJ, Leinwand LA. Functional divergence of the sarcomeric myosin, MYH7b, supports species-specific biological roles. J Biol Chem. 2023 Jan;299(1):102657. doi: 10.1016/j.jbc.2022.102657. Epub 2022 Nov 9. PMID: 36334627; PMCID: PMC9800208.

McAdow J, Yang S, Ou T, Huang G, Dobbs MB, Gurnett CA, Greenberg MJ, Johnson AN. A pathogenic mechanism associated with myopathies and structural birth defects involves TPM2-directed myogenesis. JCI Insight. 2022 Jun 22;7(12):e152466. doi: 10.1172/jci.insight.152466. PMID: 35579956; PMCID: PMC9309062.

Papadaki M, Kampaengsri T, Barrick SK, Campbell SG, von Lewinski D, Rainer PP, Harris SP, Greenberg MJ, Kirk JA. Myofilament glycation in diabetes reduces contractility by inhibiting tropomyosin movement, is rescued by cMyBPC domains. J Mol Cell Cardiol. 2022 Jan;162:1-9. doi: 10.1016/j.yjmcc.2021.08.012. Epub 2021 Sep 3. PMID: 34487755; PMCID: PMC8766917.

Barrick SK, Greenberg MJ. Cardiac myosin contraction and mechanotransduction in health and disease. J Biol Chem. 2021 Nov;297(5):101297. doi: 10.1016/j.jbc.2021.101297. Epub 2021 Oct 9. PMID: 34634306; PMCID: PMC8559575.

Wong NR, Mohan J, Kopecky BJ, Guo S, Du L, Leid J, Feng G, Lokshina I, Dmytrenko O, Luehmann H, Bajpai G, Ewald L, Bell L, Patel N, Bredemeyer A, Weinheimer CJ, Nigro JM, Kovacs A, Morimoto S, Bayguinov PO, Fisher MR, Stump WT, Greenberg M, Fitzpatrick JAJ, Epelman S, Kreisel D, Sah R, Liu Y, Hu H, Lavine KJ. Resident cardiac macrophages mediate adaptive myocardial remodeling. Immunity. 2021 Sep 14;54(9):2072-2088.e7. doi: 10.1016/j.immuni.2021.07.003. Epub 2021 Jul 27. PMID: 34320366; PMCID: PMC8446343.

Barrick SK, Greenberg L, Greenberg MJ. A troponin T variant linked with pediatric dilated cardiomyopathy reduces the coupling of thin filament activation to myosin and calcium binding. Mol Biol Cell. 2021 Aug 19;32(18):1677-1689. doi: 10.1091/mbc.E21-02-0082. Epub 2021 Jun 23. PMID: 34161147; PMCID: PMC8684737

Clippinger SR, Cloonan PE, Wang W, Greenberg L, Stump WT, Angsutararux P, Nerbonne JM, Greenberg MJ. Mechanical dysfunction of the sarcomere induced by a pathogenic mutation in troponin T drives cellular adaptation. J Gen Physiol. 2021 May 3;153(5):e202012787. doi: 10.1085/jgp.202012787. PMID: 33856419.

Bailey AL, Dmytrenko O, Greenberg L, Bredemeyer AL, Ma P, Liu J, Penna V, Winkler ES, Sviben S, Brooks E, Nair AP, Heck KA, Rali AS, Simpson L, Saririan M, Hobohm D, Stump WT, Fitzpatrick JA, Xie X, Zhang X, Shi PY, Hinson JT, Gi WT, Schmidt C, Leuschner F, Lin CY, Diamond MS, Greenberg MJ, Lavine KJ. SARS-CoV-2 Infects Human Engineered Heart Tissues and Models COVID-19 Myocarditis. JACC Basic Transl Sci. 2021 Feb 26. doi: 10.1016/j.jacbts.2021.01.002. Epub ahead of print. PMID: 33681537; PMCID: PMC7909907.

Lavine KJ, Greenberg MJ. Beyond genomics-technological advances improving the molecular characterization and precision treatment of heart failure. Heart Fail Rev. 2021 Mar;26(2):405-415. doi: 10.1007/s10741-020-10021-5. Epub 2020 Sep 3. PMID: 32885327; PMCID: PMC7897225.

Greenberg MJ, Tardiff JC. Complexity in genetic cardiomyopathies and new approaches for mechanism-based precision medicine. J Gen Physiol. 2021 Mar 1;153(3):e202012662. doi: 10.1085/jgp.202012662. PMID: 33512404.

Blackwell T, Stump WT, Clippinger SR, Greenberg MJ. Computational Tool for Ensemble Averaging of Single-Molecule Data. Biophys J. 2021 Jan 5;120(1):10-20. doi: 10.1016/j.bpj.2020.10.047. Epub 2020 Nov 26. PMID: 33248132.

Porter JR, Meller A, Zimmermann MI, Greenberg MJ, Bowman GR. Structural dynamics of isolated myosin motor domains encode differences in their mechanochemical cycles. eLife. 2020 May 29;9:e55132. PMID: 32479265.

Ezekian JE, Clippinger SR, Garcia JM, Yang Q, Denfield S, Jeewa A, Dreyer WJ, Zou W, Fan Y, Allen HD, Kim JJ, Greenberg MJ, Landstrom AP. Variant R94C in TNNT2-Encoded Troponin T Predisposes to Pediatric Restrictive Cardiomyopathy and Sudden Death Through Impaired Thin Filament Relaxation Resulting in Myocardial Diastolic Dysfunction. J Am Heart Assoc. 2020 Mar 3;9(5):e015111. doi:10.1161/JAHA.119.015111. Epub 2020 Feb 26. PubMed PMID: 32098556.

Clippinger SR, Cloonan PE, Greenberg L, Ernst M, Stump WT, Greenberg MJ. Disrupted mechanobiology links the molecular and cellular phenotypes in familial dilated cardiomyopathy. Proc Natl Acad Sci U S A. 2019 Sep 3;116(36):17831-17840. doi: 10.1073/pnas.1910962116. Epub 2019 Aug 19. PubMed PMID: 31427533; PubMed Central PMCID: PMC6731759.

Barrick SK, Clippinger SR, Greenberg L, Greenberg MJ. Computational Tool to Study Perturbations in Muscle Regulation and Its Application to Heart Disease. Biophys J. 2019 Jun 18;116(12):2246-2252. doi:10.1016/j.bpj.2019.05.002. Epub 2019 May 7. PubMed PMID: 31126584; PubMed Central PMCID: PMC6588827.

Porter JR, Moeder KE, Sibbald CA, Zimmerman MI, Hart KM, Greenberg MJ, Bowman GR. Cooperative Changes in Solvent Exposure Identify Cryptic Pockets, Switches, and Allosteric Coupling. Biophys J. 2019 Mar 5;116(5):818-830. doi: 10.1016/j.bpj.2018.11.3144. Epub 2019 Jan 25. PubMed PMID: 30744991; PubMed Central PMCID: PMC6400826.

Woody MS, Greenberg MJ, Barua B, Winkelmann DA, Goldman YE, Ostap EM. Positive cardiac inotrope omecamtiv mecarbil activates muscle despite suppressing the myosin working stroke. Nat Commun. 2018 Sep 21;9(1):3838. doi: 10.1038/s41467-018-06193-2. PubMed PMID: 30242219; PubMed Central PMCID: PMC6155018.

Greenberg MJ, Daily NJ, Wang A, Conway MK, Wakatsuki T. Genetic and Tissue Engineering Approaches to Modeling the Mechanics of Human Heart Failure for Drug Discovery. Front Cardiovasc Med. 2018 Sep 19;5:120. doi: 10.3389/fcvm.2018.00120. eCollection 2018. Review. PubMed PMID: 30283789; PubMed Central PMCID: PMC6156537.

Greenberg MJ, Shuman H, Ostap EM. Measuring the Kinetic and Mechanical Properties of Non-processive Myosins Using Optical Tweezers. Methods Mol Biol. 2017;1486:483-509. doi: 10.1007/978-1-4939-6421-5_19. PubMed PMID: 27844441; PubMed Central PMCID: PMC5506543.

Woody MS, Lewis JH, Greenberg MJ, Goldman YE, Ostap EM. MEMLET: An Easy-to-Use Tool for Data Fitting and Model Comparison Using Maximum-Likelihood Estimation. Biophys J. 2016 Jul 26;111(2):273-282. doi: 10.1016/j.bpj.2016.06.019. PubMed PMID: 27463130; PubMed Central PMCID: PMC4968482.

Greenberg MJ, Arpağ G, Tüzel E, Ostap EM. A Perspective on the Role of Myosins as Mechanosensors. Biophys J. 2016 Jun 21;110(12):2568-2576. doi: 10.1016/j.bpj.2016.05.021. PubMed PMID: 27332116; PubMed Central PMCID: PMC4919425.

Greenberg MJ, Lin T, Shuman H, Ostap EM. Mechanochemical tuning of myosin-I by the N-terminal region. Proc Natl Acad Sci U S A. 2015 Jun 30;112(26):E3337-44. doi: 10.1073/pnas.1506633112. Epub 2015 Jun 8. PubMed PMID: 26056287; PubMed Central PMCID: PMC4491760.

Greenberg MJ, Shuman H, Ostap EM. Inherent force-dependent properties of β-cardiac myosin contribute to the force-velocity relationship of cardiac muscle. Biophys J. 2014 Dec 16;107(12):L41-L44. doi: 10.1016/j.bpj.2014.11.005. PubMed PMID: 25517169; PubMed Central PMCID: PMC4269798.

Shuman H, Greenberg MJ, Zwolak A, Lin T, Sindelar CV, Dominguez R, Ostap EM. A vertebrate myosin-I structure reveals unique insights into myosin mechanochemical tuning. Proc Natl Acad Sci U S A. 2014 Feb 11;111(6):2116-21. doi: 10.1073/pnas.1321022111. Epub 2014 Jan 27. PubMed PMID: 24469830; PubMed Central PMCID: PMC3926069.

Greenberg MJ, Lin T, Goldman YE, Shuman H, Ostap EM. Myosin IC generates power over a range of loads via a new tension-sensing mechanism. Proc Natl Acad Sci U S A. 2012 Sep 11;109(37):E2433-40. doi: 10.1073/pnas.1207811109. Epub 2012 Aug 20. PubMed PMID: 22908250; PubMed Central PMCID: PMC3443183.