Date Presenter Paper 1/17/19 Introduction Eric Galburt van Oene MM, Dickinson LE, Cross B, Pedaci F, Lipfert J, Dekker NH. Applying torque to the Escherichia coli flagellar motor using magnetic tweezers. Sci Rep. 2017 Mar 7;7:43285. doi: 10.1038/srep43285. Erratum in: Sci Rep. 2018 May 18;8:46980. PubMed PMID: 28266562; PubMed Central PMCID: PMC5339722. 1/24/19 Victoria Ismail Chen Y, Zhang L, Graf L, Yu B, Liu Y, Kochs G, Zhao Y, Gao S. Conformational dynamics of dynamin-like MxA revealed by single-molecule FRET. Nat Commun. 2017 May 26;8:15744. doi: 10.1038/ncomms15744. PubMed PMID: 28548099; PubMed Central PMCID: PMC5458555. 1/31/19 Min Kyung Shinn Li Y, Yang Y, Zhang CY. Visualization and Quantification of Sortase Activity at the Single-Molecule Level via Transpeptidation-Directed Intramolecular Förster Resonance Energy Transfer. Anal Chem. 2018 Nov 6;90(21):13007-13012. doi:10.1021/acs.analchem.8b03716. Epub 2018 Oct 17. PubMed PMID: 30350606. 2/7/19 Melanie Ernst Yadav R, Lu HP. Revealing dynamically-organized receptor ion channel clusters in live cells by a correlated electric recording and super-resolution single-molecule imaging approach. Phys Chem Chem Phys. 2018 Mar 28;20(12):8088-8098. doi: 10.1039/c7cp08030a. Epub 2018 Mar 8. PubMed PMID: 29517089. 2/14/19 Sarah Clippinger Liu C, Kawana M, Song D, Ruppel KM, Spudich JA. Controlling load-dependent kinetics of β-cardiac myosin at the single-molecule level. Nat Struct Mol Biol. 2018 Jun;25(6):505-514. doi: 10.1038/s41594-018-0069-x. Epub 2018 Jun 4. PubMed PMID: 29867217; PubMed Central PMCID: PMC6092189. 2/21/19 Jackie Garcia Yasui M, Hiroshima M, Kozuka J, Sako Y, Ueda M. Automated single-molecule imaging in living cells. Nat Commun. 2018 Aug 3;9(1):3061. doi:10.1038/s41467-018-05524-7. PubMed PMID: 30076305; PubMed Central PMCID:PMC6076334. 2/28/19 Upasana Mallimadugula Zosel F, Mercadante D, Nettels D, Schuler B. A proline switch explains kinetic heterogeneity in a coupled folding and binding reaction. Nat Commun. 2018 Aug 20;9(1):3332. doi: 10.1038/s41467-018-05725-0. PubMed PMID: 30127362; PubMed Central PMCID: PMC6102232. 3/7/19 Matt Lew 3/14/19 Drake Jensen Young G, Hundt N, Cole D, Fineberg A, Andrecka J, Tyler A, Olerinyova A, Ansari A, Marklund EG, Collier MP, Chandler SA, Tkachenko O, Allen J, Crispin M, Billington N, Takagi Y, Sellers JR, Eichmann C, Selenko P, Frey L, Riek R, Galpin MR, Struwe WB, Benesch JLP, Kukura P. Quantitative mass imaging of single biological macromolecules. Science. 2018 Apr 27;360(6387):423-427. doi:10.1126/science.aar5839. PubMed PMID: 29700264; PubMed Central PMCID: PMC6103225. 3/21/19 Kacey Mersch Min D, Jefferson RE, Qi Y, Wang JY, Arbing MA, Im W, Bowie JU. Unfolding of a ClC chloride transporter retains memory of its evolutionary history. Nat Chem Biol. 2018 May;14(5):489-496. doi: 10.1038/s41589-018-0025-4. Epub 2018 Mar 26. PubMed PMID: 29581582; PubMed Central PMCID: PMC6038805. 3/28/19 Rahul Chadda Kreutzberger AJB, Ji M, Aaron J, Mihaljević L, Urban S. Rhomboid distorts lipids to break the viscosity-imposed speed limit of membrane diffusion. Science. 2019 Feb 1;363(6426). pii: eaao0076. doi: 10.1126/science.aao0076. PubMed PMID:30705155; PubMed Central PMCID: PMC6368390. 4/4/19 Jasmine Cubuk Basu S, Needham LM, Lando D, Taylor EJR, Wohlfahrt KJ, Shah D, Boucher W, Tan YL, Bates LE, Tkachenko O, Cramard J, Lagerholm BC, Eggeling C, Hendrich B, Klenerman D, Lee SF, Laue ED. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018 Jun 28;9(1):2520. doi: 10.1038/s41467-018-04486-0. PubMed PMID: 29955052; PubMed Central PMCID: PMC6023872. 4/11/19 Nicole Fazio Wasserman MR, Alejo JL, Altman RB, Blanchard SC. Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation. Nat Struct Mol Biol. 2016 Apr;23(4):333-41. doi: 10.1038/nsmb.3177. Epub 2016 Feb 29. PubMed PMID: 26926435; PubMed Central PMCID: PMC4821728. 4/18/19 Linxuan Hao Graham JE, Marians KJ, Kowalczykowski SC. Independent and Stochastic Action of DNA Polymerases in the Replisome. Cell. 2017 Jun 15;169(7):1201-1213.e17. doi:10.1016/j.cell.2017.05.041. PubMed PMID: 28622507; PubMed Central PMCID:PMC5548433. 4/25/19 Eric Tomko Wang S, Han Z, Libri D, Porrua O, Strick TR. Single-molecule characterization of extrinsic transcription termination by Sen1 helicase. Nat Commun. 2019 Apr 4;10(1):1545. doi: 10.1038/s41467-019-09560-9. PubMed PMID: 30948716.